ThisAbstract has been presented in Diabetes Association Meeting in San Antonio( Texas) , U.S. 9-13 June 2000.
本摘要已于2000年6月9日至13日在美国圣安东尼奥（德克萨斯州）糖尿病协会会议上发表。
HypoglycemicEffects of Chlorella pyrenoidosa in Streptozocin-induced Diabetic Mice.MEI-FEN, SHIH.
蛋白核小球藻对链脲佐菌素诱导的糖尿病小老鼠的降血糖作用。施美芬。
Chlorellahas been a popular foodstuff in Japan and Taiwan. Administration of Chlorella containing diet to animalsshowed some beneficial effects, e.g. lowered cholesterol levels in serum and in the liver and boosted immune . Inaddition, acute administration of Chlorella pyrenoidosa produced a significant hypoglycemic effect in alloxanizedrats. However, its acute effects onglucose and insulin response in streptozocin-induced animals are not known.
小球藻在日本和台湾是一种很受欢迎的食品。给动物喂食含小球藻的食物显示出一些有益的效果，例如降低血清和肝脏中的胆固醇水平，增强免疫。此外，蛋白核小球藻急性给药对四氧嘧啶大老鼠有显著的降血糖作用。然而，它对链脲佐菌素诱导的动物葡萄糖和胰岛素反应的急性影响尚不清楚。
Micereceived 60mg/ kg (i.p.) of Streptozocin (STZ) in citrate ( 10 mM, pH 4.8)as STZ mice or buffer only as controlmice. Basal blood glucose (BGL): blood glucose measured in samples of tail vein blood collected 60 minprior to any treatment and at 0, 30, 60, 90 and 120 min thereafter. Insulin sensitivity test: bothSTZ and control mice were treated with 100 mg/ kg of Chlorella pyrenoidosa (oral) 60 min prior tosoluble insulin (2.5IU/ kg, i.p.) and at 60 min intervals thereafter.
小鼠接受60mg/ kg（i.p.）以枸橼酸盐（10mM，pH4.8）中链脲佐菌素（STZ）作为STZ小鼠或仅以缓冲液作为对照小鼠。基础血糖（BGL）：在任何治疗前60分钟和治疗后0、30、60、90和120分钟采集的尾静脉血样本中测量的血糖。胰岛素敏感性试验：STZ和对照组小鼠在可溶性胰岛素（2.5iu/ kg，i.p.）前60min，每隔60min口服蛋白核小球藻100mg/ kg。
TheBGLs in STZ mice were significantly suppressed at 30, 60, and 90 min(+27, 164+45, 133+28mg/dL accordingly) after the acute treatment of Chlorellacompared to that at-60 min (30239 mg/dL) . In normal mice, Chlorella produced a significant but transient decreaseon BGL at 90 min( 82+7, p< 0. 05I-test, mg/dL at-60min) after the treatment.Chlorella also enhanced the hypoglycemic effects ofexogenous insulin in STZ mice. This enhancement was maintained up to 240 (+43, 317+34mg/dLat-60 min) after the insulin treatment. The same treatment did not produce significant difference in normal mice(1377at-60 min and 133 +12mg/ dLat 240min).
与-60min（30239mg/dL）相比，STZ小鼠经小球藻急性治疗后30min、60min和90min（+27164+45133+28mg/dL）BGLs明显抑制。正常小鼠治疗后90min（82+7，p<0.05i-test，mg/dL-60min）时，小球藻对BGL有明显的但短暂的降低作用，并能增强外源性胰岛素对STZ小鼠的降血糖作用。胰岛素治疗后，这种增强维持在240（+43317+34mg/dL，在-60min）。同样的处理在正常小鼠（1377at-60min和133+12mg/dL，240min）中没有产生显著差异。
Thecurrent data show that acute Chlorella pyrenoidosa administration ameliorateshyperglycemic status of STZ diabeticmice that can be maintained for as long as 3 hours after the treatment. ChronicChlorella pyrenoidosa intake may have a role in assisting blood glucose controlin diabetes. Further studies on thisissue are undergoing.
目前的数据表明，蛋白核小球藻急性给药可改善STZ糖尿病小鼠的高血糖状态，这种状态可在治疗后维持3小时。长期摄入蛋白核小球藻可能有助于糖尿病患者的血糖控制。关于这个问题的进一步研究正在进行中。